Rab27a Regulates the Peripheral Distribution of Melanosomes in Melanocytes
نویسندگان
چکیده
منابع مشابه
Rab27a Regulates the Peripheral Distribution of Melanosomes in Melanocytes
Rab GTPases are regulators of intracellular membrane traffic. We report a possible function of Rab27a, a protein implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal. We studied endogenous Rab27a and overexpressed enhanced GFP-Rab27a fusion protein in several cultured melanocyte and melanoma-derived cell lines. In p...
متن کاملRab27a and MyoVa are the primary Mlph interactors regulating melanosome transport in melanocytes.
Melanosome transport in melanocytes is a model system for the study of cytoskeletal regulation of intracellular transport. Melanophilin (Mlph) is a Rab27a- and myosin Va (MyoVa)-binding protein that regulates this process. Using yeast two-hybrid screening, we identified MT plus-end binding protein (EB1) as a melanocyte-expressed Mlph-interacting protein. To address the role of EB1 versus Rab27a...
متن کاملRab27a enables myosin Va-dependent melanosome capture by recruiting the myosin to the organelle.
The peripheral accumulation of melanosomes characteristic of wild-type mouse melanocytes is driven by a cooperative process involving long-range, bidirectional, microtubule-dependent movements coupled to capture and local movement in the actin-rich periphery by myosin Va, the product of the dilute locus. Genetic evidence suggests that Rab27a, the product of the ashen locus, functions with myosi...
متن کاملThe role of Rab27a in the regulation of melanosome distribution within retinal pigment epithelial cells.
Melanosomes within the retinal pigment epithelium (RPE) of mammals have long been thought to exhibit no movement in response to light, unlike fish and amphibian RPE. Here we show that the distribution of melanosomes within the mouse RPE undergoes modest but significant changes with the light cycle. Two hours after light onset, there is a threefold increase in the number of melanosomes in the ap...
متن کاملRab27a: A Key to Melanosome Transport in Human Melanocytes
Normal pigmentation depends on the uniform distribution of melanin-containing vesicles, the melanosomes, in the epidermis. Griscelli syndrome (GS) is a rare autosomal recessive disease, characterized by an immune deficiency and a partial albinism that has been ascribed to an abnormal melanosome distribution. GS maps to 15q21 and was first associated with mutations in the myosin-V gene. However,...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2001
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.152.4.795